DAPI derivative: a fluorescent DNA dye that can be covalently attached to biomolecules.
نویسندگان
چکیده
The preparation of a DAPI (4',6-diamidino-2-phenylindole) derivative is described. The resulting derivative retains the fluorogenic property upon binding to double-stranded DNA. Its ability for bioconjugation through amide linkage is demonstrated.
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DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of the development of this dye as a biological stain are des...
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ورودعنوان ژورنال:
- Bioorganic & medicinal chemistry letters
دوره 13 24 شماره
صفحات -
تاریخ انتشار 2003